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Literature summary extracted from

  • Hayashi, K.; Yasuda, K.; Yogo, Y.; Takita, T.; Yasukawa, K.; Ohta, M.; Kamakura, M.; Ikushiro, S.; Sakaki, T.
    Sequential hydroxylation of vitamin D2 by a genetically engineered CYP105A1 (2016), Biochem. Biophys. Res. Commun., 473, 853-858.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.22 expression in Escherichia coli Streptomyces griseolus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.22 R73A/R84A variant is also capable of converting vitamin D2 to its active form, that is, 1alpha,25-dihydroxyvitamin D2, via 25-hydroxyvitamin D2, with its 1alpha-hydroxylation activity toward 25-hydroxyvitamin D2 being much lower than that toward 25-hydroxyvitamin D3. The substitutions enhance both 25- and 26-hydroxylation activity toward vitamin D2. After 25-hydroxylation of vitamin D2, further hydroxylation at C26 may occur frequently without the release of 25-hydroxyvitamin D2 from the substrate-binding pocket Streptomyces griseolus
1.14.15.22 R73V/R84A variant is also capable of converting vitamin D2 to its active form, that is, 1alpha,25-dihydroxyvitamin D2, via 25-hydroxyvitamin D2, with its 1alpha-hydroxylation activity toward 25-hydroxyvitamin D2 being much lower than that toward 25-hydroxyvitamin D3. The substitutions enhance both 25- and 26-hydroxylation activity toward vitamin D2. After 25-hydroxylation of vitamin D2, further hydroxylation at C26 may occur frequently without the release of 25-hydroxyvitamin D2 from the substrate-binding pocket Streptomyces griseolus

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.22 Streptomyces griseolus P18326
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.22 25-hydroxyvitamin D2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Streptomyces griseolus 1alpha,25-dihydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O reaction of mutants R73A/R84A and R73V/R84A ?
1.14.15.22 25-hydroxyvitamin D2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Streptomyces griseolus 25,26-dihydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O reaction of mutants R73A/R84A and R73V/R84A ?
1.14.15.22 25-hydroxyvitamin D3 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
 Streptomyces griseolus 1alpha,25-dihydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.22 vitamin D2 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Streptomyces griseolus 25-hydroxyvitamin D2 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.22 vitamin D3 + O2 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Streptomyces griseolus 25-hydroxyvitamin D3 + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.22 CYP105A1
-
 Streptomyces griseolus